What Does Each Tube Color Indicate When Collecting Blood?

Everyone is familiar with blood collection tubes. When you go to the hospital to draw blood, you will definitely use blood collection tubes. The problem is that there are so many colors of blood collection tubes, what are the different items, and what are the differences? Let me introduce you to the blood collection tubes. Classification and meaning of different color test tube caps .

        Red test tube cap blood collection tube (additive-free dry vacuum tube)

The blood collection tube does not contain any additives, which is suitable for routine biochemical serum testing and has no effect on the test results. Disadvantages: insufficient centrifugation or insufficient incubation time, it is easy to form fibrin, and it is easy to block the pinhole of the equipment .

        Orange red test tube cap blood collection tube (coagulation tube)

A coagulant is added to the blood collection tube, which can activate fibrin and promote soluble fibrin to form a stable fibrin clot. The collected blood can be quickly centrifuged, which is generally suitable for some emergency experiments in hospitals .

        Purple test tube cap blood collection tube (the blood collection tube containing EDTA and its salts)

Purple cap, ethylenediaminetetraacetic acid (EDTA, molecular weight 292) and its salts are aminopolycarboxylic acids that can effectively chelate calcium ions in blood samples, chelating calcium or removing calcium-reactive sites will hinder Stop and stop the endogenous or exogenous coagulation process, thereby preventing blood samples from coagulating. It is suitable for general hematological tests, not suitable for coagulation test and platelet function test, nor for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test.

        Green test tube cap blood collection tube (heparin anticoagulant tube)

Also known as heparin tube, green cap, blood collection tube contains heparin sodium (lithium), heparin directly has the effect of antithrombin, adding heparin lithium anticoagulant to the inert separation rubber tube can achieve the purpose of rapid separation of plasma. The best choice for electrolyte testing, which can prolong the coagulation time of specimens, is generally suitable for routine biochemical and emergency plasma testing, and has a certain impact on some special biochemical items, and is not suitable for blood coagulation and routine blood tests . It can also be used for routine plasma biochemical testing and emergency plasma biochemical testing such as ICU. Plasma samples can be loaded directly on the machine and are stable for 48 hours under refrigeration.

        Yellow test tube cap blood collection tube (the blood collection tube containing inert separating gel and coagulant)

Inert separation gel is added to the blood collection tube. After the sample is centrifuged, the separation gel can completely separate the serum and blood cells in the blood, and the storage time is long, which is suitable for emergency serum biochemical detection .

        Black test tube cap blood collection tube (sodium citrate ESR test tube)

Generally used for blood coagulation mechanism detection, sodium citrate is less toxic and is also suitable for blood preservation .

        Light blue test tube cap blood collection tube (sodium citrate coagulation test tube)

Light blue cap, sodium citrate acts as an anticoagulant mainly by chelating calcium ions in the blood sample. Applicable to coagulation experiments, the anticoagulant concentration recommended by the National Committee for Clinical Laboratory Standards (NCCLS) is 3.2% or 3.8% (equivalent to 0.109mol/L or 0.129mol/L). The ratio of agent to blood is 1:9.

        Gray test tube cap blood collection tube (potassium oxalate/sodium fluoride)

Gray head cover, sodium fluoride is a weak anticoagulant, usually combined with potassium oxalate or sodium ethiodate, the ratio is 1 part of sodium fluoride and 3 parts of potassium oxalate. 4mg of this mixture can make 1ml of blood not coagulate and inhibit glycolysis within 23 days. It is a good preservative for blood glucose determination, and cannot be used for the determination of urea by urease method, nor for the determination of alkaline phosphatase and amylase. Recommended for blood sugar testing.

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